University of Manchester

Characterising morphology and porosity of tissue engineering scaffolds by X-ray CT

Julia Behnsen

Scientific Case

Tissue engineering is an emerging therapy for treating patients undergoing tissue trauma or disease. However, much optimisation is needed before this therapy can be implemented. Assessing the efficacy of tissue-engineered constructs is a key area to be improved in order to assist translation of these regenerative medicine products.

There are several factors of the constructs that need to be analysed to gain information on the functionality of the construct but the one feature we want to examine is the scaffold morphology, porosity and interconnectivity

Experiment Design

2-3 mm FoV of two samples
Scanners and Rigs
Xradia VersaXCT
Not Required

Sample & Safety

We wish to examine two samples, one loaded with cells the other as manufactured. This will just be the scaffold, a porous composite of collagen and hydroxyapatite. A co-culture of primary human stem cells, osteoblasts (bone cells), endothelial cells and adipose derived stem cells will be seeded (placed) onto these 10mm cylinder scaffolds.

Cells will be fixed and samples in sealed containers.
Low Hazard

Scan Records

Project Report